Properties of Fructose 1,6-Diphosphate Aldolases from Spores and Vegetative Cells of Bacillus cereus

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Properties of fructose 1,6-diphosphate aldolases from spores and vegetative cells of Bacillus cereus.

Fructose 1,6-diphosphate aldolase from cells of Bacillus cereus appears to be typical Class II aldolase as judged by its functional and physical properties. Spore and vegetative cell aldolase had similar enzymatic, immunochemical, and heat resistance properties in the absence of calcium, but they differed in their thermal stabilities in the presence of calcium, their Stokes' radii, their mobili...

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Properties of Fructose 1,6-Diphosphate Aldolases from Spores and Vegetative Cells of Bacillus cereus1

Fructose 1,6-diphosphate aldolase from cells of Bacillus cereus appears to be typical Class II aldolase as judged by its functional and physical properties: Spore and vegetative cell aldolase had similar enzymatic, immunochemical, and heat resistance properties in the absence of calcium, but they differed in their thermal stabilities in the presence of calcium, their Stokes' radii, their mobili...

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Properties of purine nucleoside phosphorylases from spores and vegetative cells of Bacillus cereus and their modification by orthophosphate.

The purine nucleoside phosphorylases (PN-phosphorylase) from spores and vegetative cells of Bacillus cereus were each purified to electrophoretic homogeneity. The turnover numbers for the spore and vegetative cell enzymes were 145 and 181 moles of inosine cleaved per mole of enzyme per set, respectively. The PN-phosphorylase from vegetative cells was more anionic than that from the spores durin...

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Bacillus cereus spores

In some Bacillus species, manganese levels influence the resistance properties of spores. To determine if this was true for Bacillus cereus, bacteria were sporulated with different MnCl2 concentrations resulting in spores with 30-fold differences in core Mn 2+ levels. Spores with different Mn 2+ levels displayed no differences in resistance to dry heat, UV radiation, γ-radiation, or hydrogen pe...

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Microgermination of Bacillus cereus spores.

The biphasic nature of germination curves of individual Bacillus cereus T spores was further characterized by assessing the effects of temperature, concentration of germinants, and some inorganic cations on microgermination. Temperature was shown to affect both phases of microgermination as well as the microlag period, whereas the concentration of l-alanine and supplementation with adenosine ex...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1969

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.98.3.1208-1218.1969